; Patel, T.R. 2015;32(5):170413. Pharmaceut Sci Tech. NCI CPTC Antibody Characterization Program. In this work, the human cadaver skin was treated to confirm the skin regeneration after the application of the microporation device. Please enable it to take advantage of the complete set of features! Although, intrinsic viscosity is a molecular parameter that can be interpreted in terms of molecular conformation, it does not offer as high resolution on molecular structure as other methods such as Light Scattering, Circular Dichroism, Sedimentation Velocity, Sedimentation Equilibrium, Size Exclusion Chromatography Coupled to Multi-Angle Laser Light Scattering (SEC-MALLS), High-performance Size Exclusion Chromatography (HPSEC), Gel Permeation Chromatography (GPC), NMR, X-rays, etc. Radiofrequency with microneedling. Web54 we estimated that the hydrodynamic radii of 3-5 kDa dextran, 20 kDa dextran, and 70 kDa dextran are 2 55 nm, 5 nm, and 6 nm, respectively. HSE and DK helped to porate the skin and to measure TEWL. Bahary, W. S.; Hogan, M. P.; Jilani, M.; Aronson, M. P. (1995). The author thanks Universidad Nacional de San Luis (Project 2-81/11), FONCyT (PICT 2004-N23-2548 and PICT 2008-N21-84), and CONICET (PIP 6324 Res. The value of P increases from 1.0272 to 1.2694 (spherical shape with tendency to spheroid for high Mw). ; Grishchenko, A.E. Dextran standards with sequence molecular weight from 8.8, 40, 71.9, 110, 200, 580 and 2000 kDa were utilized for the plot of calibration curve. Ibrahim O, Munavalli GS, Dover JS. WebDextran 670 GPC Standard Normative Mw - 670000 Da GPC standards can be used for calibration and molecular weight determination. Experimental Review on Intrinsic Viscosity Measurements and Temperature Effect. Webdextran fraction and the final product depend on changes in the hydrodynamic volume of the molecules after substitution. Biomaterials. 2008;26(11):12618. sharing sensitive information, make sure youre on a federal The exponent a is a function of polymer geometry, and varies from 0.5 to 2 and exponent is dimensionless. Deng H, Konopka CJ, Prabhu S, Sarkar S, Medina NG, Fayyaz M, Arogundade OH, Vidana Gamage HE, Shahoei SH, Nall D, Youn Y, Dobrucka IT, Audu CO, Joshi A, Melvin WJ, Gallagher KA, Selvin PR, Nelson ER, Dobrucki LW, Swanson KS, Smith AM. Progr. Weissella: An Emerging Bacterium with Promising Health Benefits. Advances in Cosmetic Surgery. The same way, many of these authors emphasize the non-linear or hyperbranched of dextran molecular weight range from 9 to 2000kDa [3,5,8,16, 37,38]. This site needs JavaScript to work properly. Synthesis and characterization of blue dextrans. There is a formula that used to calculate the radius (in Angstrom) from the molar mass (in g/mol) of dextran, a=0.33*(MM^0.46) where, a and MM are Terms and Conditions, The Mark-Houwink parameters and the effect of temperature on them are specifically studied. Ann Surg Oncol. The 70-kDa FITC dextran reached the jejunum after 15 min and passed the entire small intestine within 1 h after its administration, demonstrating that 4-kDa FITC ; Rjumtsev, E.I. government site. TdB Labs produce a wide range of FITC-derivatives (490 nm/520 nm) with a mean molecular weight ranging from 4 kDa to 2000 kDa. Table1 shows TEWL values measured immediately after the microporation of human and pig skins. (2008). On the molecular weight distribution of dextran T-500. Durand, A. Armstrong, J. K.; Wenby, R. B.; Meiselman, H. J.; Fisher, T. C. (2004). Pathan IB, Setty CM. Dynamics of a Polymer Solution in a Rigid Matriz. The analysis of errors using the Solomon-Ciuta method is compared with HM [59]. These studies were again focused on investigating the hydrodynamic properties of the polymer molecules, their hydrodynamic radius in relation to their radius of gyration and the coherence of the simulation results with polymer theory. Unperturbed dimensions and theta temperature of dextran in aqueous solutions. 2006;3(3):37385. A few percent of R (13) glucosidic linkages provides side chains which appear to be short. The most widely used method for the characterization of macromolecules is the capillary viscometry, as it is a simple and economic method. Dynamic light scattering study confirmed the mono-disperse nature of dextran with hydrodynamic radius of 900 nm. (more or less, with a hydrodynamic radius of less than 100 nm) to cross from the vasculature into the interstitium. 2.It can be found from the figure that at the start-up stage of the shaft, the hydrodynamic effect (HE) is weak, so the HP is not enough to support the shaft, which The skin culture medium was purchased from Biopredic International (Saint-Grgoire, France). Data File Dextran. Walkenhorst, S.; Olivier, R. (2003). The regeneration process of the skin is regulated by growth factors such as keratinocyte growth factor and fibroblast growth factor, which trigger the proliferation and migration of cells [23, 24]. FITC-derivatives have been used for a variety of biological applications for more than 40 yeras. This situation is mainly the result of using dextans, solvent, additives, etc. Using the polymer standards, a plot of the ln [] vs ln Mw usually gives a straight line. Ther. In the case of a poor solvent, a temperature increase causes an increase in entropy and intrinsic viscosity and is favorable for the extended conformation. Cecopieri-Gmez, Martha L.; Palacios-Alquisira, Joaqun (2005). Ionic Polymers Based on Dextran: Hydrodynamic Properties in Aqueous Solution and Solvent Mixtures. Dextran; Weissella cibaria; exopolysaccharide; prebiotic; skimmed milk. (1998). Radiofrequency ablation of malignant liver tumors. For 40 kDa molecules, the hydrodynamic diameter dh ~8 nm is much smaller than the collagen mesh size [ 33, 41 ]. Palmuti Braga Vettoria, Mary Helen; Martins Franchetti, Sandra Mara; Contiero, Jonas (2012). This low polydispersity realizes that the Mark-Houwink equation is forced at a given Mw regardless of the hyperbranched nature of the dextran molecule. Carbohydrate Polymers 87, 405-410. These values indicate for dextrans in dilute aqueous solution, both methods give very similar results of the intrinsic viscosity and the respective Mw. Carbohydrate Polymers, 68, 477-488. "Dextrans in Aqueous Solution. @ Nilimesh See attached. eCollection 2018 Feb. Molecules. (1990). (2008). As intrinsic viscosity of dextrans decreases with an increase in solution temperature; chain flexibility enhances with an increase in temperature (see Table 4) showing that the intrinsic viscosity of dextran (with different molecular weights Mw from 8.8 to 200kDa) decreases linearly with an temperature increase in solution, but change to high molecular weight to hyperbranched macromolecule. The Solomon-Ciuta single-point method is used for calculating dextran intrinsic viscosity and the error relative is calculated, and %RE respect to Huggins method is >4.18% for 2000kDa, and >2.06% for 8.8kDa (Table 2). Biological skin substitutes for wound cover and closure. Chen Rong Huei; Min Lang Tsai (1998). FITC-dextran molecular weights; 4, 10, and 20kDa molecular weight were purchased from Sigma (St. Louis, MO, USA). The human and pig skin samples were placed in the receiver chamber of the Transdermal diffusion cell system with the SC facing up and the donor chamber was fixed in place. Unauthorized use of these marks is strictly prohibited. European Polymer Journal, 36, 2143-2146. SWC designed and coordinated the research. The cumulative permeation of FITC-dextran 10 K was 4.82 0.32 M, in the human skin and 5.92 0.46 M in the pig skin. The optical image and histological analysis confirmed that the micropores on the skin were recovered over time. J Pharm Sci. 2004;25(9):155967. J. The polymer molecule assumes a tighter configuration, and the solution has a lower intrinsic viscosity. However, when molecules deviate from globular to non-spherical or elongated structures then Rg/Rh tend to values upwards of The study of biopolymers as dextran in aqueous solution, is effectively determined by intrinsic viscosity [] measurements at different temperatures. Radiofrequency is a well-known medical technique with expanded application in electrosurgery for laparoscopic medical procedures and in dermatology, for sebaceous glands ablation in acne treatment [12, 13], and treatment of photoaging skin symptoms, including wrinkles and hyperpigmentation [14]. The great deviation at 2000kDa occurs due to the large hyperbranched structure of the macromolecule, generating a change in its conformation and, therefore, a change in the way it flows [73]. Pamis, R.; Hernndez Cifre, J. G.; Lpez Martnez, M.C. Table 5 shows the data of Mark-Houwink parameters determined in figure 4 from linear regressions with 2 values higher than 0.9797. Hi Khanh, Alan is taking a little time off of Research Gate at the moment, but here is some information in reply to your question. These results could be attributed to the differences in the in vivo and in vitro skin nutrients supply; with in vivo studies, the skin tissue is supplied nutrients directly from the blood vessels, but the human cadaver skin is supplied from the media, therefore, the skin tissue regeneration efficiency is low [26,27,28]. Monosaccharide analysis revealed that the polysaccharide comprised only glucose units. A solvent at this temperature is called a theta solvent [53]. dextran hydrodynamic radius. The most widely applied coatings for FDA-approved SPIONs are dextran and carboxydextran. Immediately after formation, the microchannels are filled with interstitial fluid, making them hydrophilic, and a suitable drug delivery system [15]. Biophysical Journal, 87, 4259-4270. Anal. Table 2 compares the properties of nanoparticles coated with one or other polymer. Just in case anyone wants to use a different molecular weight to estimate the hydrodynamic size: the calculator is integrated into the classic Zeta Markierte Dextrane sind hydrophile Polysaccharide, die hufig in mikroskopischen Studien zur berwachung der Zellteilung, zum Verfolgen der Bewegung von lebenden Zellen und zur Untersuchung der hydrodynamischen Eigenschaften der zytoplasmatischen Matrix verwendet werden. Carbohydrate Polymers 72, 356-360. All authors have consented to the submission of this manuscript for publication. International Journal of Pharmaceutics, 277, 99-104. International Journal of Biological Macromolecules, 42, 133-137. International Journal of Biological Macromolecules, 23, 135-141. Therefore, the trend of "a" and kM-H decreases with T, where the presence of ethylene glycol or dimethylsulfoxide strongly changes the interactions of this solvent with the macromolecule. Intrinsic viscosity molecular weight relationship. Journal of Physical Chemistry. ; Tsuchiya, H.M.; Rist, C.E. The Transdermal diffusion cell system (DHC-6TD, LOGAN instrument, USA) was used to assess FITC-dextran permeability in vitro. (2003). The cumulative permeation of FITC-dextran 10K was 4.820.32M, in the human skin and 5.920.46M in the pig skin. TEWL measurements were taken 30min after microporation of the human and pig skin. 1905/05) for their financial support. CAS The biological activity of cationic liposomes in drug delivery and toxicity test in animal models. Not for use in diagnostic procedures. WebFluorescein isothiocyanatedextran average mol wt 60,000-76,000; CAS Number: 60842-46-8; Synonyms: FITCDextran; find Sigma-Aldrich-FD70S MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich Banga AK. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. Keywords: intrinsic viscosity, dextrans, Mark-Houwink parameters, hydrodynamic, temperature, Journal of Polymer and Biopolymer Physics Chemistry, 2013 1 (1), Keywords: FITC dextran; intestinal permeability; transit time. American Journal of Food Science and Technology 2013, 1(3), 60-66. (1954). Beer, M.U. a Microscopy images of micropore and b quantified data using ImageJ after 0 (immediately), 4, 8, and 24h according to the number of times microproration was done (n=150, *: p<0.05). Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Microporation was performed 2, 5, and 10 times on each sample. Pharm Res. The Dextrans intrinsic viscosity is determined as a function of the molecular weight in water and NaOH 0.5 M see reference [1]. The numerical value of a indicates that dextrans acquire the shape of a rigid sphere in aqueous solution; and kM-H demonstrates that under water the value decreases with temperature [71, 72]. SEC of mono-carboxymethyl cellulose (CMC) in a wide range of pH, MarkHouwink constants. Hydrodynamic properties, such as the intrinsic viscosity, [], and equilibrium solution properties such as the hydrodynamic radius RH can be combined to construct dimensionless quantities that are universal since they are of being independent of the macromolecular particle size, while they depend more or less sensitively on its shape [51]. Dilute solution properties and degree of chain branching for dextran. The solution viscosity () is related to the solvent viscosity (o), resulting in the relative viscosity. Thus, typically, the dextran glomerular for a molecule with a Stokes-Einstein (SE) radius of 30 is approximately sevenfold higher than that of neutral horseradish Typical examples are the classical size-independent combinations like the Flory parameter, which that combine the intrinsic viscosity, [], and the gyration radius, Rg: These quantities have been proposed along the years, by different eminent scientists, after whom they are named. Eremeeva, T. E.; Bykova, T. O. Before Harding, S. E.; Varum, K.; Stoke B.; Smidsrod, O. 2015;23(3):2239. dextran hydrodynamic radius. Carbohydr Polym. The explanation for the anomalous values of a and kM-H is because the mixture solute-solvent is highly compatible due a great compaction of biopolymer, from undisturbed state in the absence of interactions, where free energy of mixing less than zero, and emphasize the nature non-linear or hyperbranched of dextran for Mw high to 200kDa. Where f0 is the frictional coefficient of a sphere having the same hydrodynamic (hydrated or solvated) volume V as the particle. 2009;301(8):60913. A single frequency laser is directed to the sample contained in a cuvette. The (a/b) value is 2.5 which confirms that dextran in aqueous solution is a biopolymer with a spherical conformation for an Mw range from 8.8 to 200kDa, and with tendency to compaction with increasing temperature (RH decreases). It is worth noting that the size of macromolecules is much bigger than that of solvent (water) molecules [50]. Curley SA. Expert Rev Med Devices. In vivo iontophoretic delivery of salmon calcitonin across microporated skin. Carbohydrate Polymers, 36, 319-326. 2023 BioMed Central Ltd unless otherwise stated. 8. As well as being able to measure hydrodynamic radius in the Zetasizer (if you have access to one), it's also provided with a calculator that allows 10% neutral buffered formalin was purchased from Hisko (Gunpo, Korea). This universalization of the parameters leads to a higher error ratio in the calculation of the respective molecular weights. Leiva, Angel; Muoz, Natalia; Urza, Marcela; Gargallo, Ligia; Radic, Deodato (2010). Many works are aimed at determining molecular weights [1, 7, 26], hydrodynamic radius, among other parameters for characterizing the physics and chemistry properties of dextran in solution. Challenges for the modern analytical ultracentrifuge analysis of polysaccharides. Kloster, C.; Bica C.; Rochas C.; Samios, D.; Geissler, E. (2000). Progress in Polymer Science, 30, 38-70. Doke SK, Dhawale SC. The .gov means its official. Human cadaver skins (fresh human full-thickness skin disc, 12~20mm) were supplied by Biopredic International (Saint-Grgoire, France). Characterization of high molecular weight dextran produced by Weissella cibaria CMGDEX3. 2018;1:10915. The genus Weissella: taxonomy, ecology and biotechnological potential. An official website of the United States government. The Principle of the Suspended Level: Applications to the Measurement of Viscosity and Other Properties of Liquids. Rotureau, E.; Dellacherie, E.; Durand, A. Transdermal drug delivery system for non-steroidal anti inflammatory drugs: a review. Huggins, Maurice L. (1942). osmotic pressure or light scattering). The values of Mark-Houwink parameters universalized for the range of working temperatures are the following: 0.4267 for a*, and 0.1455 cm3/g for k*M-H, with 2 of 0.9399. After the pore closure examination, each sample was stored in a 10% neutral buffered formalin awaiting haematoxylin/eosin (H&E) staining.
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